Scientific Instrumentation

Due to Evergreen moving to remote learning for Spring Quarter 2020, we will not be holding in-person workshops for the foreseeable future. Additionally, use of the instruments is not possible at this time.

Inorganic Analysis

Atomic Absorption Spectrometer (AAS)

The AAS is used for qualitative and quantitative elemental analysis.

Sample Types: water, soil, minerals, airborne particulates and biological materials

Detection Limit: ~0.2 – 10 ppm depending on analyte

Inductively Coupled Plasma – Mass Spectrometer (ICP-MS)

The ICP-MS is used for qualitative and quantitative multi-elemental analysis, especially trace elemental analysis.

Sample Types: water, soil, minerals, airborne particulates and biological materials

Detection Limit: ~ppt (parts per trillion)

Mercury Analyzer

Nippon Instruments Corporation MA 3000

The mercury analyzer is used for qualitative and quantitative determination of mercury.

Sample Types: water, soil, minerals, airborne particulates and biological materials

Detection Limit: ~0.002 ng

Contact

Abir Biswas X6433

Sina Hill X6466

Carbon, Hydrogen, Nitrogen Analyzer (C, H, N)

PerkinElmer Series II CHNS/O Analyzer 2400

The CHN analyzer is used for qualitative and quantitative analysis of the carbon, hydrogen, and nitrogen content of a sample.

Sample types: soil; water, and biological materials

Detection Limit: ~ .001 mgs

Contact

Dylan Fischer X6509

Diane Nelsen X6472

Discrete Analyzer

Seal Analytical

The discrete analyzer is used for quantitative and qualitative analysis of anions and cations.

Sample Types: water, soil, and biological materials

Detection Limit: ~0.06-0.2 ppm depending on analyte

Contact

Carri LeRoy X5483

Trisha Towanda X6472

Ion Chromatograph (IC)

The IC is used for qualitative and quantitative analysis of anions and can analyze several analytes in the sample run. 

Sample types: fresh water (no brackish or saltwater), water extracted soil air particulates

Detection Limit: ~100 ppb

Polarograph

The polarograph is used for qualitative and quantitative analysis of anions, cations, functional groups in organic compounds, and elemental analysis. It can also measure redox conditions.

Sample types: water, soil, airborne particulates, and biological materials

Detection Limit: ~ppb depending on analyte

 

Organic Analysis

Fourier Transform Nuclear Magnetic Resonance Spectrometer (FT-NMR)

Oxford 400 Mz

The FT-NMR is used to for qualitative and quantitative analysis of organic compounds, and is most commonly used to determine the carbon-hydrogen framework of organic molecules.

Sample Types: organic compounds

Detection limit: ~µM

Contact

Lydia McKinstry X5262

Diane Nelsen X6472

Gas Chromatograph Mass Spectrometer (GC-MS)

The GC-MS is used for qualitative and quantitative analysis. It can separate components of a mixture and provide information about the chemical structures of compounds

Sample types: organic liquids, gases, soil, water, and biological materials

Detection limits: ~ ppb

Fourier Transform Infrared Spectrometer (FT-IR)

The FT-IR is used for qualitative and quantitative analysis of organic and inorganic compounds. and most commonly is used to identify functional groups within a compound.

Sample types: organic and inorganic compounds.

Detection limits: ~

 

Microscopes

Stereomicroscopes

Leica MZ16

Leica MZ16 Training Video  (This video is out of date, but provides a basic overview of microscope functions)

This stereomicroscope is suitable for life science and materials research. This microscope uses proprietary Leica LAS X software, allowing for expanded focal imaging (or Z-stacking) capability and advanced image analysis. It is equipped with two illumination sources (transmitted and side light) and images are captured using a high-resolution color camera (Leica MC190 HD).

Sample Types:  Best suited for sample that are too large or opaque for a compound microscope. Can be used for wet or dry samples. 

Magnification: 5-115X​

Contact

Dan Cygnar X6470 (cygnard@evergreen.edu)

Leica M205 FCA

This stereomicroscope is suitable for life science and materials research. This microscope uses proprietary Leica LAS X software, allowing for expanded focal imaging (or Z-stacking) capability and advanced image analysis. This is a semi-motorized microscope and is equipped with multiple illumination sources (transmitted, ring light, relief-contrast, darkfield, side light), has epi-fluorescence capability (DAPI and GFP filter sets), and images are captured using a high-resolution color camera.

Sample Types: Best suited for sample that are too large or opaque for a compound microscope. Can be used for wet or dry samples.

Magnification: 7.8X to 320X (not all illumination techniques available at maximum magnification)

Contact

Trisha Towanda X6472 (towandat@evergreen.edu)

Dan Cygnar X6470 (cygnard@evergreen.edu)

Erik Thuesen X6584 (ThuesenE@evergreen.edu)

Upright Compound Microscope

Olympus BX63

This compound microscope is suitable for life science research. It is an upright, fully motorized microscope optimized for slide samples. This microscope uses proprietary Olympus cellSens software allowing for easy operation, image acquisition and analysis. It is equipped with several imaging methods and high-resolution dual sensor (color/monochrome) camera, allowing for both color and high sensitivity (low signal) data collection.

Imaging methods: Brightfield, phase contrast, differential interference contrast, epi-fluorescence (DAPI, FITC, TRITC filter sets)

Sample types: Best suited for slide mounted samples or microtome sectioned tissue

Magnification: 40-1000X

Contact

Dan Cygnar X6470 (cygnard@evergreen.edu)​

Pauline Yu X6860

Inverted Compound Microscope

Olympus IX81

This compound microscope is suitable for life science research.  It is an inverted, fully motorized microscope optimized for live cell analysis, cell culture flasks or suspension samples (not slides).  This microscope uses proprietary Olympus cellSens software allowing for easy operation, image acquisition and analysis.  It is equipped with several imaging methods and two cameras (color or monochrome) for color and high sensitivity (low signal) data collection.

Imaging methods: Brightfield, differential interference contrast, epi-fluorescence (DAPI, FITC, TRITC, Cy-5), confocal (spinning disk)

Sample types:  Cell culture flasks, specimen dishes, or deep well slides/plates.  Slide mounted sample can be observed, but not optimally.

Magnification: 40-1000X

Contact

Dan Cygnar X6470 (cygnard@evergreen.edu)

Trisha Towanda X6472 (towandat@evergreen.edu)

Scanning Electron Microscope (SEM)

JEOL JSM-6480LV

The SEM is an electron microscope that provides morphological, topographical, and compositional information about the surface of the specimen.

Sample types: Fixed, dried, and coated samples

Magnification: 8-300,000X

Contact

Trisha Towanda X6472 (towandat@evergreen.edu)

Dan Cygnar X6470 (cygnard@evergreen.edu)

Gerardo Chin-Leo X6514